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A majority of these long-lived deletion mutants contained in the set of 564 randomly selected deletion strains have been previously described [15]; however, here we report three novel long-lived strains (inp51Δ, msw1Δ, and rpl37bΔ) that show increased RLS in both mating types (Figure 4).

Using the native state as the prevalent form and focusing on the early events, we have shown here that combining the observations from all-atom simulations of ten PrP pathogenic mutants contained in the α2-α3 region, depicts a common perturbation pathway that is supported by previous unrelated experimental findings.

The fast scenario, also in contradiction with observations, predicts that proportion of mutants contained in expansions of intermediate sizes markedly decreases with age (Supplemental Note 6).

Therefore in a last refinement round both GAG-binding sites, the proximal loop and the C-terminal helix, were engineered simultaneously which resulted in a further significant improvement of the GAG-binding affinities for these mutants (all mutants contained, in addition, the Δ6 N-terminal deletion).

Similar(56)

The Δhly DP-L21611; [55]), ΔactA DP-L30788; [56]) and ΔplcAB DP-L19366; [46]) mutants contained in-frame deletions of the respective genes in the 10403s background.

Mutants containing in-frame deletions of the clpB and capB genes, respectively, of SCHU S4 have been previously described [21].

Three classes of mutant contained in-frame chromosomal deletions in this region and one mutant class contained a short duplication.

A mutant lacking PGA29 is not currently available, but the pga30−/− mutant contained in our library, was tested and did not display any of the pga31−/− phenotypes.

Whole-genome sequencing confirmed that the four suppressor mutations were induced independently because each suppressor mutant contained, in addition to the specific suppressor mutation, hundreds of additional unique mutations.

All R. sphaeroides mutants we constructed contained in-frame markerless deletions.

Mutants contained changes in the zinc finger region, such as R366C, H377Y, and R394W, failed to activate the Cyclin E1 promoter.

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