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For a given H-ChC sample, the percentage of mutations contained in each signature is consistent between HCC and iCCA (Fig. 4a).
The resulting signatures thus provide a succinct summary of H2H adaptive mutations contained in any influenza proteome.
It is noteworthy that the mutations contained in the mutants we obtained occur in all three domains, which are postulated to exhibit protease, DNA recognition, and small molecule sensoring functions, respectively.
According to PANTHER, 14% of missense mutations contained in the database are predicted to be deleterious to protein function.
According to PolyPhen, 20% of missense mutations contained in the database are predicted to be deleterious (either 'possibly' or 'probably' damaging) to protein function.
Finally mutations contained in this list were manually verified using Tablet Genome viewer [ 54] by looking up the mutation positions in the BAM files generated by gsMapper.
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Since the causal mutation contained in the BH2 haplotype is still not known, the status of the animals was based on the haplotype and can be prone to errors with respect to carrying or not the underlying mutation.
Nearly all tumors with mutations contained them in the lin−CD49f+CD24+ and lin−CD49f−CD24+ subpopulations.
Both tumours with KIT mutations contained the hotspot mutation L576P in exon 11 (Table 2).
The mutations were contained in the BlpI/SacI fragment, and the sequence of this fragment was verified for each mutant by automated sequencing at the University of Virginia Biomolecular Research Facility.
A breakdown of the different types of mutations currently contained in the database is also shown in Figure 2B.
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