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These mutants confirmed the influence of cell membrane composition on cyanobacterial tolerance to short-chain alcohols.
Expression of PTEN mutants confirmed the lipid-phosphatase activity as critical, while co-expression of a dominant-negative form of Akt overcame the PTEN shRNA effect.
LC-MS analysis of the purified GFP mutants confirmed the selective incorporation of the unnatural amino acid in all cases (Figure S1, Figure S2, Figure S3, Figure S4 and Figure S5).
Nucleotide sequencing of the 1679-bp fragment of the five mutants confirmed the presence of the in-frame deletion.
A study involving deletion mutants confirmed the role of the C-terminus substrate-binding domain (EEVD) of Hsp70 and amino acids 128 165 of M1 for this association.
Western blot analysis of Tup1p and Cyc8p levels in the respective cyc8 and tup1 deletion mutants confirmed the stability of each subunit was unaffected by the absence of the other (Fig. 2D and Supplemental Fig. S1).
Similar(52)
These μ mutants confirm the role and specificity of residues that line the Yxxφ-binding pocket of μ1 in the interaction with MHC-I CD-Nef.
A similar overproliferation phenotype is evident in clones of α-Adaptin mutants, confirming the specificity of the RNAi line.
In continuous light, all AtCHR23 mutants confirm the pattern of root length as presented above for long-day conditions.
X-ray crystal structure determinations of selected mutants confirm the structure of the remolded active site contour and illuminate the binding mode of the analogue BTAC.
Scopoletin content was dramatically decreased in knock-out cyp98a3 mutants, confirming the role of the Arabidopsis C3'H in the synthesis of this compound.
Related(15)
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genes confirmed the
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mutants depolarized the
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