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Experimental data on these mutants supported the assumption.
When expressed in a sugar transporter-deficient strain of S. cerevisiae, selective mutants supported the co-consumption of xylose and glucose in mixed sugar fermentations using industrially relevant sugar concentrations.
Shieh and Angaroni suggested that the majority of helical missense mutants supported the synthesis of reduced levels of G6PC protein compared to the wild-type enzyme, indicating these mutations destabilize G6PC [ 1, 8].
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Our identification of apparently identical tooth phenotypes in Lrp4 and Wise mutants supports the premise that Lrp4 has a role in the modulation of Bmp signalling.
Finally, the virulence defects we observed for the UTI89 ΔhofQ and CFT073 ΔyheF ΔhofQ mutants support the idea that the K12-homologous T2SS and T4P gene clusters are functional and expressed under conditions encountered within the host.
Considering the results obtained with PaHyPRE mutants supporting the key role of Cys88 and Cys236 residues in catalysis and the large overall structural similarity with TcPRAC, our data supports a reaction mechanism similar to PRAC where HyPRE equally possesses two active sites per dimer, each one including two catalytic Cys.
Importantly, we find that a deletion mutation in ubc-13 that results in an early nonsense codon (and therefore is likely a null; Figure S5) causes the same GLR-1 trafficking defects as observed in uev-1 mutants, supporting the idea that a UEV-1/UBC-13 heterodimer complex is regulating GLR-1 trafficking.
Recent studies on autophagy-specific atg gene mutants support the involvement of autophagy in plant immune responses [ 6, 8].
Experimental studies with these mutants support the behavior of the simulated networks and the involvement of the efflux of anions at the plasma membrane in the process [ 6- 9].
The reduction of catalytic activity in the Tyr204 mutants supports the suppression of an ionization mechanism involving the hydroxyl of Tyr204.
The discordant phenotypes of the F137X and F233X mutants support the idea that gating charge pathways are different at this particular position.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com