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In this study we have made pairwise combinations among some of the best mutants at each of these 6 sites.
In this study we have purified improved mutants at each of these positions and determined their steady-state kinetic parameters for CB1954 and for the antibiotic nitrofurazone.
Significant differences in the proportion of mutants at each time point were evaluated by one-tailed Fisher's exact tests, with significance level = 0.05.
To implement this experimentally, we assayed 435 mutants at each generation.
The fluorescence intensity and lifetime decay were measured as described above for each of the mutants at each of the three pH conditions at 20 °C.
We expressed serine mutants at each site (N182S, N239S and N298S) with or without the K308A mutation to determine how each site was utilized.
Similar(49)
The tri3 expression was not detected in the Δtri3 mutant at each time point.
The tri4 expression was not detected in the Δtri4 mutant at each time point (Fig. 4).
The relative enrichment of each mutant at each position provides information on its contribution to protein activity or ligand-binding under the conditions of the screen.
2 3 embryos from each group (control or mutant) at each different embryonic stage (E5.5, E6.5 or E7.5) were used.
The probability to create a double mutant at each division is proportional to the probability that a 1-hit mutant (and not a wild-type) cell divides.
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