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Percentages indicate relative amounts of variants at each stage.
From a pooled sample of many people's DNA, the scientists cloned tiny DNA snippets representing the common variants at each spot, creating a library of such snippets.
A consensus sequence would be built making a note of all the observed variants at each position.
The number of sequence variants at each position, weighted by the number of reads that the variant occurred in was tabulated and converted into graphical representations of nucleotide counts at each position in the OTU.
Variants at each HVR have been frequently exchanged across bacterial strains, generating highly chimeric proteins [ 31].
Figure 5a shows the similarity between populations in terms of shared variants at each position of the miRNA precursor compared.
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Only one variant at each locus was chosen and only individuals with complete genotype data at all eight variants were included in the obesity-risk-allele score analyses.
First, the most frequent variant at each position is usually a transition rather than a transversion, although several positions did not follow this pattern.
In contrast with the pharmacogenetic literature, in which the term allele is used to indicate protein variants and corresponding DNA sequences regardless of the number of mutations, we use the term allele to indicate the variant at each nucleotide position and the term haplotype to indicate the overall sequence.
This difference is significant in most cases (Fig. 6), indicating that selection acts to maintain the appropriate motif variant at each particular location.
To finalise the consensus sequence, the most common variant at each SNP was checked by mapping the Illumina HiSeq reads to the candidate repeats using bwa-mem [ 54], extracting variant frequencies from the Samtools mpileup file [ 58] using VarScan 2 [ 59].
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