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While our data do not rule out a slight increase in mtDNA oxidative damage, comparison with the ageing cohort study clearly shows that mtDNA oxidative damage levels in young SOD mutants are lower than those seen in old WT animals (Figure 4C and 6C).

In Figure 7C, the mutants are lower because we used the membrane fraction for the proteinase K protection assay.

However, the ratio changes of AFD neurons in 23°C-cultivated crh-1 tz2) crh-1 tz2re lower than those in 23°C-cultivated wild-type animutantsig 5C).

Note that the TFEα expression levels are higher than TFEβ, and that the expression levels of TFEβ mutants are lower than WT, which in some cases make it difficult to discern in the input fractions.

A study of intramolecular ET by pulse radiolytically produced disulfide radicals to Cu II) in the above rationally designed azurin mutants showed that the reorganization energies of the designed mutants are lower than that of WT azurin, increasing the intramolecular ET rate constants almost 10-fold.

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Moreover, the optimum pHs of the G276K, G276Y, and G276N mutants were lower than that of the wild type.

The rate of emergence of phage-resistant mutants was lower for phage phT4A when compared with phage ECA2 and phage cocktail phT4A/ECA2.The results indicate that in addition to the efficacy, the potential development of phage-resistant mutants must also be considered in the design of phage cocktails.

However, the numbers of infective juveniles produced on all mutants were lower than that on the wild type (Fig. 4B), and no infective juveniles were produced on purL−.

As expected, the caspase activities in both the cmp1Δ and crz1Δ mutants were lower than that in wild-type cells (Fig. 4B).

It is noteworthy that the fertilization success rate of the mutants was lower than that of the controls, suggesting there may be some defects in the mutant oocytes that affected their fertilization.

The expression levels of both F857-L mutants were lower than the wild type constructs, but no significant difference in the S1/S2 cleavage was observed (Fig. 3b, lane 9 12).

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