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Phloem transport and unloading is not changed in the shortest roots of the pear quintuple mutant, which are strongly affected in the radial growth.
We used two different dnEps15 mutants and one control mutant, which are all fused to GFP.
To confirm this result, we measured the expression level of fgf20a in the medusa/sdf1a mutant which are adult viable [28].
All of the single-cysteine mutants in the sequence 107 135 were derivatives of this mutant, which are labeled as P107C R135C/Basal mutant.
As Arg344 is involved in cation-pi interaction with Phe4, the appearance of peak-A after 5 min of incubation clearly suggests that Arg344 is much more exposed and susceptible to proteolytic digestion by trypsin in ΔF4 and F4A mutants compared to BSX and the F4W mutant, which are tolerant to trypsin proteolytic digestion.
To analyze the inner dynein arm components, the dyf13 mutant was crossed with the oda1 mutant, which are missing outer dynein arms (Kamiya, 1988).
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In this work, we report specific behavioral, cognitive, and sleep homeostatic defects in the after hours (Afh) circadian mouse mutant, which is characterized by lengthened circadian period.
In contrast, the IncEcm F125A mutant, which is defective in its interaction with SNX5, did not interfere with the CI-MPR transport (Figures 4c and d).
TFEB S142A and S138A mutants showed highly impaired export kinetics, whereas the S211A mutant, which is efficiently phosphorylated on S142 and S138 (Fig. 4c), retained, by enlarge, its ability to be exported.
As expected, the escN null mutant, which is a T3SS ATPase mutant, exhibited very low translocation ability, due to its inability to secrete and translocate effector proteins (Fig. 6B).
The S. typhimurium A1-R mutant, which is auxotrophic for leu arg, is tumor-seeking and has antitumor efficacy against the major types of cancer.
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