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The second cross was done in the same way, but with fewer lines: for the ceob2 mutant, we analysed three plates each of three independent lines; for the trt-1 mutant, we analysed eight plates of one line; and for the trt-1/ceob2 mutant, we analysed eight plates each of three different lines.
To examine the extent of fluorophore labelling on the V216C mutant, we analysed the mass values of the V216C mutant before and after labelling with fluorescein-5-maleimide using ESI MS.
To further dissect the phenotype observed in the cenp-metaΔ single mutant, we analysed living cenp-metaΔ neuroblasts by time-lapse microscopy to determine the mitotic timing (i.e. the time that elapses between Nuclear Envelope Breakdown (NEB) and anaphase onset).
As for the dag1gai-t6 double mutant, we analysed by PCR-based genotyping more than one hundred F2 plants derived from both the dag1 × gai-t6 and the reciprocal cross, but we were unable to isolate the dag1gai-t6 double mutant.
Similar(56)
To determine if any residual or fused Hsp90α protein persists in gene disruption mutants, we analysed the same tissues from homozygous mutant mice.
In the subset of mutants we analysed we found a total of 38 new WD40 repeat units.
To investigate the contribution of the failure in phagosome processing to lamellipodial and motility defects in SCAR Δ37 mutants we analysed SCAR Δ37 ;H99 double mutants, which lack all apoptosis.
The study by Changolkar et al. was performed using a fixed mutation on the C57Bl6 background whereas the mutants we analysed had a genetic background composed of half 129Ola and half C57Bl6 (intercrosses of the F2 generation).
To characterize the glycosylation state of WT ACE and the mutants, we analysed ACE in cell lysates and culture medium after treatment with either PNGase F (peptide: N-glycosidase F) or Endo H (endoglycosidase H).
In light of the impaired secretion of amelogenin and ameloblastin in M100888 mutant mice, we analysed the effects of WT amelogenin, Y64H mutant amelogenin and ameloblastin expression, both singly and in combination, in a transfected eukaryotic cell line of epithelial origin.
Using antibodies to the conserved N-termini of predicted mutant proteins, we analysed MSI-High colorectal cancer cell lines for examples of naturally expressed mutant proteins arising from frameshift mutations in coding microsatellites (cMS) by immunoprecipitation and Western Blot experiments.
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