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In order to investigate the mechanism of the high efficiency of the pDC line, we analysed changes in the activation level of the pDCs.
After activating this particular signaling cascade in a BL cell line we analysed respective gene expression profiles of IKKa, IKKb, TRAF2, TRAF6, Jak3, BCL-3 and p38 deficient cells.
As Dicer-deficient ES cells are not capable of differentiation and we have not been able to isolate a stable Dicer-deficient XX ES cell line, we analysed Dicer-deficient XX embryos produced by mating Dicerwt/Δ heterozygous mice.
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We detected an increase in putrescine levels in all the lines we analysed irrespective of whether major or minor changes in ADC activity were observed.
To characterize the properties of the isolated cell lines, we analysed alkaline phosphatase activity, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 and Nanog by immunostaining.
In contrast to the strong cytotoxicity toward allogeneic LCLs exhibited by the T cell lines we analysed, the pp65/A*0201-specific CD8 T cells cross-reactive to allogeneic molecules described by Gamadia et al. were not able to kill allogeneic LCLs, though they proliferated and produced IFN-γ against allogeneic LCL and killed autologous LCL loaded with the pp65/A2 peptide [15].
Due to the observed permanent tyrosine phosphorylation of Jak2 and Tyk2 in cHL cell lines we analysed cHL cell lines for the presence of activating mutations.
To determine the hierarchical organisation of breast cancer cell lines, we analysed the tumourigenic potential of the CD24−/low/CD44+ and CD24+/CD44+ cell populations of HCC1954 and MCF7 cell lines.
To investigate the mechanisms responsible for downregulation of CKMT1 gene expression in OSCC-derived cell lines, we analysed genomic DNA by PCR-SSCP andirectct sequence analyses.
As the differential response to chemotherapy could be the result of different growth rates in the cell lines, we analysed the proliferation rates of the cells.
To confirm a compensatory upregulation of PbSERA3 in the SERAser loss-of-function parasite lines we analysed the protein levels in merozoites (Fig. 8B).
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