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The medaka pc mutant was first isolated by Dr. Hideo Tomita at Nagoya University [15] and has been maintained in a homozygous state over many generations (pc homozygotes develop into adults and are able to produce offspring).
Each Wnt3a mutant was first tested for its biological activity using a Wnt reporter assay.
The phenotype of BoOr mutant was first described in 1975 [ 37].
The vib-1 mutant was first identified in a search for mutations that alleviate heterokaryon incompatibility (HI) in N. crassa (Xiang and Glass 2002).
The spontaneous, nonlethal yellow foliage mutant was first observed in a segregating F3 population derived from a cross between M99-274166 × 'MN0091'MN0091
The structure of the designed mutant was first minimized for 500 steps using the steepest decent algorithm with the backbone of the protein restrained before being subjected to the following system setup and production runs.
Similar(51)
In RADPRE, ratios of normalized probe intensities between wild type Columbia and a pcfs4 mutant were first generated.
To test the stability of the mutants, 3×103 CFU of each mutant were first seeded on tryptic soy agar, either plain or supplemented with nalidixic acid (25 µg/ml) or kanamycin (50 µg/ml).
When a purR mutant is first plated on lactose (adenine) medium, it grows nonselectively at a rate of 2.3 hr/generation for the first 24 hr of incubation, using contaminants present in the agar (see Table 3).
To create pot-1 ; pot-2 double mutants, a pot-1 ; unc-52 double mutant and a pot-2 ; dpy-17 unc-32 triple mutant were first created; phenotypically wild-type F2 progeny of unc-52 / pot-2 ; pot-1 / dpy-17, unc-32 F1 heterozygotes were selected; and the strains that segregated only phenotypically wild-type F3 progeny were retained for analysis.
The biological activity of these mutants was first tested in a RNP replication system using a CAT gene-containing viral replicon.
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