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When AChE586-599 (50 µM) was fibrilized in the presence of various concentrations of the F3/A mutant (ranging from 1.56 µM to equimolar), some of AChE586-599 fibrilization parameters were affected.
However, matching the transcriptional profiling results, we invariably found lower levels of potassium (K) in the mutant (ranging from 1.4 3.0 fold reduction).
To establish sensitivity, serial 10-fold dilutions of the standard N1 plasmids (wild-type and mutant) ranging from 10 to 10 copies/μL were used as a template.
Pathogenesis-related protein 10 family of proteins (PR10) exhibited a significant alteration in their expression patterns in the Li 1 mutant, ranging from 8to9696 fold difference between Li 1 and WT.
Experiments using different lots of HU show variability in the severity of the HU-induced chromatin bridge defect in the smc-5 (ok2421 ) mutant, ranging from 60 to >90% frequency for this defect.
Similar(55)
The number of mutations identified in each mutant ranged from 33 to 77 (Table 2), which closely matches the number of mutations (30−64) predicted using Canavanine-resistance mutation rates in Gruber et al. (2012).
In addition, we observed considerable heterogeneity in the rest and activity phenotype in Bdr mutants ranging from robust and entrained rhythms to highly irregular circadian profiles.
The rates of deamino-NADH oxidase activity among the nineteen mutants ranged from 32 to 94% of the wild type rate.
These Met mutants range from high to low in tyrosine kinase activity [20], [21], [22] and develop a unique tumor spectrum of primarily sarcomas and lymphomas on a C57BL/6;129/SV (backgroundound [19].
Pearson correlation coefficients obtained for predictions within each of the 12 individual mutants ranged from 0.74 to 0.96.
The concentration of hTP was ∼8.8 mg/ml and that of the various hTP mutants ranged from 7.5 to 20 mg/ml.
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