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PAEC extracts were then used as a PCMT source to check whether transfection successfully produced the overexpression of the proper protein species (wild type or mutant) or, in the case of antisense, silenced the endogenous PCMT.

An occasional population of Antp>GFP cells is found in the posterior lobes of the mutant or in microtumors (M.K. and S.G., unpublished data).

However, other genetic modifications introduced during the generation of the parental double deleted mutant or in the recombinant isolation process could also account for their in vivo attenuation.

Depletion of EMB-1 either in a temperature-sensitive mutant or in RNAi mediated knockdowns results in embryos that arrest at metaphase of meiosis I.

Consistently with a common role as part of the 9-1-1 heterotrimer, rad9-d phenocopied hus1-d and rad1-d, either as a single mutant or in combination with fan1-d (Fig. 3B).

An increase in the acetylation of H4 in a sir2 (histone deacetylase) mutant or in cells overexpressing acetylase Sas2 led to increased rates of chromosome loss and to synthetic dosage lethality in kinetochore mutants.

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Similar(47)

However, the reported alleles of these genes may not be desirable in actual breeding programs because of too much reduction in tiller number of moc1 mutant or dwarfism in the high tiller number mutants.

This could be explained because ste11 mRNA levels are higher in the pka1Δ tor2-51 double mutant than in any of the single mutants or in the wild-type.

In non-legume the symbiotic-like responses were observed only in nim1/npr1 mutants, or in NahG transformants, both of which suppress the SA signaling (Fig. 1).

Nevertheless, in the case of the D136N/T143D and E129K/D136N double mutants or in the 1∶1 mixture of Cry1Ab with the double mutants, the 250-kDa oligomers were observed in the soluble fraction (Fig 2C).

The NF treatment induced root hair deformation exclusively in the SA-insensitive nim1/npr1 mutants, or in SA-deficient NahG transformants, in agreement with data observed in plants treated with intact S. meliloti (Fig. 1, NF).

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