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Exact(6)
And this she writes to me this week: "I am a mutant of two languages: English and Spanish.
We next constructed a double mutant of two subunit-encoding genes of the AP-3 complex (Δ Ncap3mΔ Ncap3mΔ.
A double mutant of two lysines, identified by lysine-scanning mutagenesis, was shown to be deficient in PIP3 binding and membrane translocation.
It was suggested that the phospho-defective mutant of two serine residues of Snail1 at Ser96 and Ser100, DS 96 GxxS 100), β-TrCP destruction motif showed an increased half-life after CHX treatment.
Though all the existing ts APC/C subunit mutants are viable and healthy at 15°, we have been unable to construct a double mutant of two APC/C subunits that could be maintained at the permissive temperature of 15° (Shakes et al. 2003; Stein et al. 2007; our unpublished observations).
Besides, the double mutant of two global regulatory families, nodD1 and nodW, was reported to be unable to induce nodY lacZ fusion in the presence of genistein or daidzein, but still showed a 2- to 3-fold induction in the presence of soybean seed extracts (SSE).
Similar(54)
To that end, we tested the N17 mutant of Hras-val12.
The phenotype of the Delta 40 bp mutant resembled to some extent the phenotype of the ORF K3 mutant of MHV-68 [37].
β3-subunit showed a significant decrease in co-precipitation efficiency from 12±3% with wild-type bestrophin-1 to 4±1% (n = 5; p = 0.035 unpaired t-test) with the ΔCT-PxxP mutant of bestrophin-1.
Genes coding the constitutively active-type of mutant of three BmRas, which were fused with GFP, were transiently introduced into Sf-9 cells and the cellular localization of their products was observed.
To explore the role of the newly detected cluster, we generated a deletion mutant of bestropin-1 lacking the proline-rich motifs between amino acid positions 462 and 575 (named ΔCT-PxxP).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com