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Nicolaides, N. C. et al. Mutations of two PMS homologues in hereditary nonpolyposis colon cancer.
Nicolaides NC, Papadopoulos N, Liu B, Wei YF, et al. Mutations of two PMS homologues in hereditary non-polyposis colon cancer.
Regarding the role of these various virulence factors identified in LF82, we further analyzed the association of pathoadaptative mutations of two of them that play a major role in the interaction of the LF82 bacteria with host intestinal cells.
Genetic interaction analysis, in which the combined mutations of two genes exhibit phenotypes significantly different from the single mutation of either one [1], is a powerful tool allowing biologists to investigate the genetic components of an organism [2].
This observation further suggests that mutations of a single gene in one signaling pathway may not necessarily lethally affect the pathway, and that dysfunctions of one pathway are often the result of the co-occurrence of mutations of two or more genes.
The kinase-dead ppk1 carrying mutations of two histidine residues (required for autophosphorylation) to alanine [17] [ppk1-KD] wamplifiedied by PCR from a suitable construct essentially as described by Sureka et al. [17] using the above primers and integrated into M. smegmatis mc2155 as described above.
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The iPLEX test included two plexes, which comprised seven mutations of the APC gene and 29 mutations of three of the mismatch repair genes.
Mutations of five amino acid residues in TEVpM2 slightly altered protein secondary structure conformed by circular dichroism assay.
Mutations of nine genes, such as FCGBP and VCAN, that have been related to the maintenance of stem state were found in all seven H-ChC patients (Supplementary Table 9).
In that group, mutations of three genes are attributed to germination dormancy.
Mutations of three genes all encoding ion-channels or membrane ionic pumps were discovered from 1996 to 2005.
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