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Doubling time and spontaneous TK mutant frequencies of the established cell lines were similar to those of the wild-type cells.

We then diluted this pool serially with unmodified RNA, to obtain mutant frequencies of 3.16%, 1.0%, and 0.316%.

The mutant frequencies of resistance to the combinations of tobramycin plus fosfomycin (4 and 128 µg/ml, respectively) and imipenem plus fosfomycin (4 and 128 µg/ml, respectively) were studied.

In contrast, the insertion-deletion mutant frequencies of the two forms were similar (data not shown) when examined using a 6-nucleotide gap-filling substrate containing a template TTTT run in the +1 reading frame.

The study was designed to determine if the elevated mutant frequencies of treated patients represented independent mutations or resulted from the in vivo expansion of single mutant cells.

For the remaining simulations, we considered selection coefficients of s = 0.2 and s = 0.5 with initial mutant frequencies of p = 0.05 and P = 0.01, respectively.

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Similar(52)

The overall mean mutant frequency of hypothalamus was also slightly but significantly higher than the mean mutant frequency of hippocampus (p = 0.0267).

It is possible that this increase is entirely due to the increase in mutant frequency of hippocampus and hypothalamus.

Combined across age, a significant difference was observed when the mean mutant frequency of hippocampus or hypothalamus were compared to the mean mutant frequency of cortex (p = 0.0305; p<0.00001), remainder (p = 0.0148; p<0.0001) or when the mutant frequency of hypothalamus was compared to total brain (p<0.0001) (Table S1).

Similarly, at 7 months of age, the hypothalamus showed a mean mutant frequency of 8.1×10−5 increasing to 14.6×10−5 in 30-month old animals (p = 0.0032).

The most extensive poliovirus mutant frequency data set estimated an average mutant frequency of 2 × 10−4 (Acevedo et al., 2014).

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