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Alcian blue staining of skeletons at E15.5 and E18.5 showed c-Myc mutant elements continued to be reduced in size and exhibited small primary ossification centers (Fig. 3A D), consistent with delayed and/or defective endochondral ossification.
In contrast, GFP expression of a reporter mRNA (GFP-3xIPTmiR-4306xmt2), containing miR-430 target sites with the mutant elements of #16 that do not bind to DAZL [15], was not largely affected by DAZL (1.0-fold, Fig. 3B).
We made several mutant elements carrying specific alterations in the Zorro3 sequence.
To begin an analysis of the sequences important for Zorro3 retrotransposition we made three more mutant elements.
The mutant elements were transformed into C. albicans and the retrotransposition frequency associated with each measured and compared to that of wild-type elements (Table 2).
These mutant elements were transformed into C. albicans and the associated retrotransposition frequency was measured and compared with that of wild-type elements (Fig. 7, Table 2).
Similar(51)
Reporters containing mutant responsive elements (REm) were constructed to serve as negative controls (Fig. 1A).
EMSAs were carried out using T. gondii nuclear extract with these radiolabeled wildtype or mutant TRP2 elements and binding pattern observed.
As expected, CT-SBP2 shifted wild-type but not mutant SECIS elements in which the AUGA motif in the SECIS core was deleted.
As a PHO box has been found overlapping the distal transcriptional starting point of pqsR and as AQ production is no longer enhanced in a phoB mutant, these elements may mediate the increased AQ production observed following phosphate limitation (Jensen et al., 2006).
In ta mutants, cartilaginous elements within the oral-facial complex are enlarged and dysmorphic (Fig. 7).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com