Sentence examples for mutant subclones from inspiring English sources

Slow growing mutant subclones were observed.

Cytogenetic analysis was performed on the HPRT-mutant subclones selected from unirradiated Chinese hamster V-79 cells and from HPRT- mutant subclones that arose after exposure to γ-rays, 1GeV protons and 14N-ions (LET - 77 keV/μm), produced by the synchrophasotron and the U-400M heavy ion accelerator.

The multifaceted activities of XPO1 inhibitors may not only enhance p53-mediated MCL cell death but also delay or prevent the selection of p53 mutant subclones during therapy.

(37) Clinical concern about p53 activators would be the selection or induction of p53 mutant subclones during therapy, which has been reported in in vitro cultures with Nutlin-3a.

Nevertheless, it has been demonstrated that the KRAS mutant subclones known to cause resistance to anti-EGFR therapy are quite prevalent and frequently occur at low levels (levels below the detection limit by DS [8]).

Of the initially characterized subclones (column one of Table 2), all of the 28 singly mutant cells formed light blue colonies on rich medium with X-gal, while the 16 doubly mutant subclones formed dark blue colonies.

Sometimes, two or more distinct areas can be distinguished within a single polyp, with cells in one area appearing relatively normal and those in the other appearing clearly cancerous, as though they have arisen as a mutant subclone within the original adenoma clone.

cDNAs coding for non-tagged human wildtype TRPC6, FLAG-tagged human wildtype TRPC6, FLAG-tagged human TRPC6 carrying the N143S mutation [10], and a cDNA coding for the FLAG-tagged, dominant-negative human TRPC6 pore mutant all subcloned in the pcDNA3 vector (Invitrogen), served as templates for site-directed mutagenesis [27].

For construction of Baz-S151E, a point mutation was introduced at the S151 residue by site-directed mutagenesis using the polymerase chain reaction (PCR), and the mutant was subcloned into pUAST-EGFP-attB.

Human GSK-3 β protein coding region (both WT and constitutively active S9A mutant) were subcloned into this pCMS4-eGFP-H1P-shGSK-3 β vector at MluI/NotI sites.

In order to confirm the effect of the T to A mutation on mRNA splicing, the wild-type minigene L1-GHRexon8-L2 and the corresponding mutant were subcloned in the mammalian pcDNA3.1 vector and transfected into HEK293 cells.