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PCR analysis of the luxS mutant confirmed that the kan gene was inserted into the luxS gene in the same orientation as the luxS gene.

Complementation testing with a known Scn8a mouse mutant confirmed that this mutation is responsible for the Cloth-ears phenotype.

Our growth assays with PtoDC3000 in the mlo2-11 (pmutant point mutant confirmed that it did not exhibit increased resistance to P. syringae (Additional file 6B).

An in vitro kinase assay with the 124A223A mutant confirmed that CK2 was no longer able to induce Par-4 phosphorylation.

The sequence of the B gene cDNA clones from the splice junction mutant confirmed that the mRNAs produced from this gene were aberrantly spliced.

Usage of a fungicidal treatment and a Magnaporthe mutant confirmed that penetration was not necessary for this early activation of CYP96B22.

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Confocal laser scanning microscopy (CLSM) revealed that the proportion of biofilm-forming cells decreased substantially in a flo11 mutant, confirming that FLO11 was responsible for biofilm formation.

In 3T3-TrkA cells, hNGFR100W and hNGFR100E were completely unable to induce the phosphorylation of PLC- γ1 with respect to hNGF (Fig. 1C), unlike other hNGFR100 mutants, confirming that hNGFR100W and hNGFR100E share similar properties.

In the related parasite Leishmania mexicana, knockout mutants confirm that arginase is required for parasite viability, so the inhibition of arginase is a validated strategy in the search for new antiparasitic agents.

Interestingly, the significant reduction in terms of cytotoxicity is not accompanied by a significant reduction in the uptake (~15 20%), binding, catabolism, processing, and presentation of immunodominant peptides to CD4+ or CD8+ T cells from these LLO mutants, confirming that antigenicity of LLO is also independent of its cytotoxic property [ 18].

The proteins encoded by each gene localize to the plasma membrane and have been shown to complement the growth defect of a yeast Fe uptake mutant, confirming that they are functional Fe transporters (Bughio et al. 2002; Ishimaru et al. 2006).

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