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To identify genes that potentially regulate the accumulation, mobilization, and transport of photoassimilates in rice (Oryza sativa L). leaves, we recently screened a mutant collection of rice by iodine staining to visualize leaf starch contents.
We constructed a mutant collection of 4023 melon M2 families.
Here, we used multi-parameter quantitative phenotype analyses of an arrayed mutant collection of yeast cells induced to proliferate peroxisomes, to establish a comprehensive inventory of genes required for peroxisome induction and function.
By screening such a mutant collection of A. thaliana, we have identified several mutants with enhanced resistance against M. persicae [ 23].
The analysis of the mutant collection of model organisms revealed that single-gene knockouts did not usually exhibit an observable phenotype [ 57- 59].
Using a systematic and comprehensive gene-disrupted mutant collection of E. coli (the Keio collection), we carried out genome-wide screenings of genes affecting glycogen metabolism in this bacterial species.
Similar(51)
When we retested the phenotype of transposon mutants found in TBCF10839 in publicly available transposon mutant collections of sequenced strains PAO1 [84] or PA14 [85], we noted that biochemical or physiological phenotypes of low complexity such as the presence of an enzyme or metabolite were similar in most, but not all tested cases (see examples in Figure 6).
The platform described here reliably provides mutant collections of unparalleled genotypic diversity and will enable a wide range of functional genomic studies in S. aureus.
The null mutant collections of 103 non-essential protein kinases (NPKs) and 28 non-essential phosphotases, created by in vivo recombination in S. cerevisiae followed by A. nidulans protoplast transformation (http://www.fgsc.net/Aspergillus/KO_Cassettes.htm; [ 49, 50]) were utilised.
Screening of mutant collections to identify inactivating mutants of spy, one of the plant OGT orthologues, showed that three of these (mapping to XcOGT D288, G291 and G293; Silverstone et al, 2007) corresponded to point mutations clustering in the mobile active site lid loop that controls access to the β-face of the sugar.
The Tos17 mutant collection consisting of approximately 50,000 mutant lines characterized into flanking sequences have been used in full characterization of many genes characterized in Japan and nearly 50 Tos17 mutant lines have been successfully used in tagging specific genes (See reference list at https://tos.nias.affrc.go.jp/doc/references.html).html
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com