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Importantly, the addition of FeSO4 to tatAyCy or total-tat mutant cells growing exponentially in LB without salt completely prevented the occurrence of the lysis phase (Figure S4).

In Table 1 we show that levQ mutant cells growing on all four of the tested sugars (glucose, fructose, mannose and galactose) produced little expression of the PlevD-cat fusion [10].

We observed that lgl mutant cells growing in wild-type imaginal wing discs show poor viability and are eliminated by Jun N-terminal Kinase (JNK -dependent cell death.

In those investigations, wild-type and mutant cells, growing in complex nitrogen-rich medium, were treated with rapamycin for 6 hr to mimic nutrient starvation.

Wildtype and mutant cells growing in synthetic medium were temperature shifted to 36°C for 1 hr and starved for methionine for 30 min. Cells were then harvested by centrifugation, resuspended in one-tenth the original volume, and pulse-labeled with 150 µCi/ml S-methionine (>600 Ci/mmol, cell-labeling grade; Perkin Elmer, Waltham, MA) for 90 s.

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At the non-permissive temperature, sec9-4 mutant cells grew very poorly and failed to form individual colonies, while sec9-4 vmutantouble mutant cells grew and formed colonies.

Both nrg1Δ and tup1Δ mutant cells grew as filaments under these conditions (Fig, 6 A).

However, we found that the quadruple sap mutant cells grow very poorly in sucrose media.

The G346E mutant cells grew extremely slowly on the regular SPP medium.

When compared to wild-type cells, sec9-7 mutant cells grew better at elevated temperatures than sec9-4 mutant cells.

We analyzed the distribution for the 18S rRNA precursors in mutant cells grown in permissive (Gal) and restrictive (Glc) conditions.

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