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Exact(5)
Importantly, the addition of FeSO4 to tatAyCy or total-tat mutant cells growing exponentially in LB without salt completely prevented the occurrence of the lysis phase (Figure S4).
In Table 1 we show that levQ mutant cells growing on all four of the tested sugars (glucose, fructose, mannose and galactose) produced little expression of the PlevD-cat fusion [10].
We observed that lgl mutant cells growing in wild-type imaginal wing discs show poor viability and are eliminated by Jun N-terminal Kinase (JNK -dependent cell death.
In those investigations, wild-type and mutant cells, growing in complex nitrogen-rich medium, were treated with rapamycin for 6 hr to mimic nutrient starvation.
Wildtype and mutant cells growing in synthetic medium were temperature shifted to 36°C for 1 hr and starved for methionine for 30 min. Cells were then harvested by centrifugation, resuspended in one-tenth the original volume, and pulse-labeled with 150 µCi/ml S-methionine (>600 Ci/mmol, cell-labeling grade; Perkin Elmer, Waltham, MA) for 90 s.
Similar(55)
At the non-permissive temperature, sec9-4 mutant cells grew very poorly and failed to form individual colonies, while sec9-4 vmutantouble mutant cells grew and formed colonies.
Both nrg1Δ and tup1Δ mutant cells grew as filaments under these conditions (Fig, 6 A).
However, we found that the quadruple sap mutant cells grow very poorly in sucrose media.
The G346E mutant cells grew extremely slowly on the regular SPP medium.
When compared to wild-type cells, sec9-7 mutant cells grew better at elevated temperatures than sec9-4 mutant cells.
We analyzed the distribution for the 18S rRNA precursors in mutant cells grown in permissive (Gal) and restrictive (Glc) conditions.
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