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However, in both sgo1-100 and sgo1-700 mutant cells progressing from G1 into the cell cycle, Brn1-6HA retained a partial ability to associate with the centromere and pericentromere, though only in the case of the sgo1-100 did this occur in a timely manner.
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Anti-apoptotic mechanisms are obvious candidates for allowing mutant cells to progress though normally restrictive bottlenecks.
One interesting observation was that the cell cycle of mutant APC8-harboring cells progressed, even with a disturbed pattern [ 45].
The mcm5 -461 temperature sensitive mutant is leaky because eventually cells progress through the cell cycle and will form colonies at 37° after several days (Leon et al. 2008).
These mutant cells failed to progress beyond the G1 phase of the cell cycle after extended time periods (96 hours exposure to DOX) even though most single unbudded cells (70%) were viable when these cells were arrayed onto YPD medium without DOX (data not shown).
Latency reflects biochemical changes required after initial radiation if mutant cells are to progress and form a tumour eventually identified as cause of death, and historical changes in environmental factors which interact with radiation for a particular cancer.
To study changes in Xlf1 phosphorylation status as cells progressed through the cell cycle, we used a temperature-sensitive cdc10 mutant cdc10-M177) to synchronize GFP- xlf1 cultures in G1.
Cell widths expanded as the cells progressed shootward along the length of the meristem for Col-0 and all mutants evaluated (Additional file 2).
We observe enrichment of mutant cells as iterations progress for all values of motility strength (k B ) (left panel).
While wild-type cells at the d/v boundary were arrested in G1, the UAS- dpn RNAi; E spl) XP mutant cells located at the ZNC progressed in the cell cycle, as monitored by EdU incorporation and by the expression of the G2/M specific marker Cyclin -B (Fig. 5E H).
Upon MSH2 depletion, however, mutant FA-J cells progress to G2/M (Fig 5E).
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