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Lysates from the wild type (WT), pafA-null mutant, and complemented dop-null mutant Mtb strains showed hydrolysis of 1 a.
Safranin staining was performed on basal stem sections of the Arabidopsis wild-type, irx10 irx10-L double mutant and complemented plants to identify regions of lignification.
Even though the C. jejuni wild-type strain, ciaD mutant, and complemented ciaD isolate resulted in greater activation of NF-κB (p65 subunit phospho-S536) compared to the flgBC mutant, significant differences were not observed in the amount of NF-κB activation between the isolates.
In the mutant and complemented plants, however, additional sites were seen, some of which were unique to either mutant or complemented plants.
We further examined the cell growth of the WT, deletion mutant, and complemented strains in THY medium with extra carbon source by supplementation with 2% (w/v) gluconate sodium (Fig. 2B).
The physiology of both mutant and complemented strains indicate that the contribution of HYDA2 to H2 photoproduction is approximately 25% that of HYDA1, which corresponds to similarly low levels of in vitro hydrogenase activity measured in the hydA1-1 mutant.
Similar(29)
To examine functions of PdeL and PdeH, conidiation of Guy11, ΔpdeL, ΔpdeH mutants, and complemented mutant strains were examined.
When the wild type, covR-deletion mutant (IBS606), and complemented covR-deletion mutant (IBS606/pIB30) strains, containing the P1882-gusA transcriptional fusion reporter, were assayed for Gus activity.
Similarly, expression of the rice OsSPA1 protein in Arabidopsis spa mutants failed to complement any phenotypes of light-grown spa mutant plants and complemented the phenotype of dark-grown seedlings at a much reduced efficiency.
We tested the mutant (1F8) and complemented (1F8 + pCNAG_07701) strains for sensitivity to copper starvation using the copper chelator bathocuproinedisulfonic acid (BCS).
Two strains, namely, Salmonella Enteritidis sefD mutant 29108 (ΔSefD 29108) and complemented mutant 100713 (SefD+ 100713) (Morales et al., 2012), were used to prepare bacterins.
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