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We observed the loss of a subset of conjugated proteins with Ufm1G98A mutant and complete loss of conjugating proteins with Ufm1ΔC (Figure S4 compare lanes 2 and 3 with lane 1).
It is currently unclear whether this discrepancy is due to cell-type specific functions of Peli1 or the differences between expression of a Peli1 mutant and complete ablation of Peli1.
Azide, fluoroaluminate, and fluoroscandium caused insignificant inhibition of αT349A, -D, and -Q mutants, slight inhibition of the αT349R mutant, partial inhibition of the αT349R/βR182A double mutant, and complete inhibition of the wild type.
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As expected, wild type and rec12Δ mutants enter and complete meiotic S phase between two and three hours.
The stamens of ISS mutants initiate and complete the early stages of development, suggesting that SPF is present.
This suggests that despite the deletion of the sec8 gene in sec8Δ1 mutants, and almost complete elimination of Sec8 in the NMJ of homozygous mutants, there is substantial maternal contribution of Sec8 in other tissues, as previously shown for other sec proteins [ 7, 8, 21- 23].
Ca2+ and, to a lesser extent, Sr2+ substantially improve growth of the double mutants on minimal and complete media such that we routinely supplement them with Ca2+ (Fig. 1A).
12) Figure 2 would be stronger if the authors included parallel studies with the W419A mutant and provided more complete analysis of the motility data.
Importantly, unlike M ybx1 single mutants, the M ybx1;sqt cz35 compound mutant embryos can initiate and complete gastrulation, and do not manifest any cytokinesis or YSL defect even at the restrictive temperature (new Figure 8C).
As shown here, the mapping of traits that distinguish any two sequenced accessions, including mutant genotypes, can be conceive and complete in six months using BC1 populations.
In these screens, suppressor alleles are selected that allow daf-2 pathway mutants to bypass dauer arrest and complete reproductive development.
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