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Overexpression or mutant analyses of the TPS genes will uncover their function.
Over-expression and mutant analyses of CBLs and CIPKs have greatly enriched our understanding of their functions.
Early mutant analyses of mouse knockouts of Hoxa1 described a delayed hindbrain neural tube closure in the anterior hindbrain, although no analysis of these defects at the cellular level has been reported (Lufkin et al., 1991; Mark et al., 1993).
This predicted outcome was confirmed in vivo by mutant analyses of leg developmental phenotypes in which the requirement to restrain N signaling was found to become more significant at higher temperature.
Several genes have also been identified in S. cerevisiae and Escherichia coli that are responsible for the reduction of furanic aldehydes (Petersson et al. 2006; Gutiérrez et al. 2006) In the mutant analyses of furanic compound-degrading microorganisms, several additional genes with similar functions were identified (Koopman et al. 2010b; Nichols and Mertens 2008).
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Double-mutant analyses of era1 and the ABA-insensitive mutants abi1 and abi2 showed a suppression of the ABA-insensitive phenotypes.
Next, we performed double mutant analyses with strong loss-of-function alleles of existing engulfment mutants of the ced-1/6/7 pathway.
Our approach extends the applicability of NGS-based mutant analyses beyond the domain of available reference genomes.
Genes containing these binding sites are enriched in the gene sets induced by over-expression of the respective FruM isoform in males, and in the genes identified as induced by FruM in loss-of-function mutant analyses.
Mutant analyses further revealed that expression of many of the genes is suppressed in dark grown plants by Phytochrome Interacting transcription Factors (PIFs) and activated by photoactivated phytochromes, which in turn degrade PIFs and mediate a coordinated induction of the genes.
Surprisingly, the cellular transcriptional response to IR in D. radiodurans appeared largely stochastic, and mutant analyses confirmed that many of the highly induced uncharacterized genes were unrelated to survival.
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Justyna Jupowicz-Kozak
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