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Parts of these effects are probably mediated by muscle tissue specific IGF-I isoforms such as the mechano growth factor [ 29].
The cDNA clones selected for full-length sequencing were from a non full-length enriched white muscle tissue specific library constructed as described in Adzhubei et al. [ 10].
High quality full-length insert sequences from 560 pre-smolt white muscle tissue specific cDNAs were generated, accession numbers [GenBank: BT043497 - BT044056].
Clones were selected from a white muscle tissue specific library from pre-smolt developmental stage since our research at the time this study was initiated also focused on discovering genes that might be important to the ability of depositing dietary cartenoid pigments in muscle tissue.
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Calpain 3 (Calso), also named p94, is a skeletal muscle tissue-specific protein known to be responsible for limb-girdle muscular dystrophy type 2A (LGMD2A).
We then integrated available information on cellular metabolic pathways and fluxes, cellular metabolic control mechanisms, catalytic enzyme kinetic mechanisms, subcellular compartmentation and metabolites volumes of distributions, inter-domain transport mechanisms, and skeletal muscle tissue-specific metabolic characteristics.
Regarding the safety issue of gene therapy using vector cassettes, muscle tissue-specific promoters are considered to be advantageous for eliminating transgene expression in other types of cells, especially antigen presenting cells [40], [41], [42], [43].
Regarding skeletal muscle, tissue-specific biological processes like muscle structure development (GO 0061061; 302 genes), muscle cell differentiation (GO 0042692; 200 genes) or muscle contraction (GO 0006936; 156 genes) were also highly represented in the annotated gilthead sea bream transcriptome.
Although the presence of D2 in skeletal muscle has long been recognized, its role has been questioned given the very low specific activity and some muscle tissue-specific confounding factors.
The SUVR appeared to depend on the existence of specific uptake, because there were no significant differences in the SUVRs between granulomas and muscle tissue where specific uptake was absent.
The aim of this chapter is to give an overview of skeletal muscle and describe some of the methods used in our laboratories and others to engineer skeletal muscle tissue, highlighting specific benefits of each model and their utility both for transplantation, as applied to the craniofacial region, and in furthering biological understanding of skeletal muscle plasticity in health and disease.
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