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In this study we expressed GFP-tagged proteins under the control of a muscle cell specific promoter and determined their sub-cellular localization using fluorescence microscopy.
We therefore investigated the effects of androgens on the testicular vasculature directly using an arteriole smooth muscle cell specific ARKO mouse (termed SMARKO) [8].
We observed expression of myo-3::GFP, which represents expression of a body wall muscle cell specific myosin [36], [37], in approximately 81% of L1 derived cultured cells (Figure 2, Table 1).
Smoothelin is a smooth muscle cell specific marker [ 16].
ACTA2 and MYH11 encode the smooth muscle cell specific α-actin and β-myosin heavy chain, respectively.
It is important to note that the viruses we used to manipulate Tnfsf14 levels in vivo were not muscle cell specific.
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SM22α-Cre transgenic mice were crossed with Atg7flox/flox mice for the generation of vascular smooth muscle cell-specific Atg7 knockout mice.
Vascular smooth muscle cell-specific Atg7 deletion leads to sarcoplasmic reticulum swelling and imbalanced Ca2+ homeostasis, resulting in altered contractility (Michiels et al., 2015).
MCK-Cre mice were crossed with Atg7flox/flox mice for the generation of skeletal muscle cell-specific Atg7 knockout (SMC-Atg7−/−) mice.
To investigate this and determine the overall importance of this mechanism in testis function, we generated a blood vessel smooth muscle cell-specific AR knockout mouse (SMARKO).
To examine how changes in the expression profiles of early mesodermal markers affect the differentiation of other lineages of mesodermal origin, we analyzed the timing and quantified the levels of expression of blood, endothelial and smooth muscle cell-specific markers.
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