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As an example, Wang et al. used Fe3O4-Au rods as optical probes for multiplex pathogen detection [6].
To date, most multiplex pathogen detection has been performed using traditional hybridization microarrays [ 8, 33].
This rapid multiplex pathogen detection system showed a higher pathogen detection rate in comparison with blood culture analysis.
The observed dynamic range is an improvement over most previously developed multiplex pathogen detection assays, where the dynamic range was often limited to 100 1000 [ 21, 32].
Circularizable ligation probes (Padlock probes) [ 20] have previously been applied successfully for multiplex pathogen detection [ 21], but do not allow quantification.
In this study, we described a high-throughput diagnostic system that combines very specific multiplex pathogen detection with accurate quantification over a range of target concentrations.
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Here we describe a novel two-step technique involving multiplex PCR pathogen detection by CE-SSCP analysis followed by singleplex PCR pathogen quantification by CE-SSCP.
Multiplex PCR pathogen detection is useful as an adjunct to blood cultures to support early adjustment of empiric antimicrobial therapy.
Nowadays, successful multiplex qPCR-based pathogen detection methodologies have been realized [ 12- 14]; however, due to a limited number of potential dyes, the attainable level of multiplexing is low [ 9, 15].
The added value of multiplex DNA amplification-based pathogen detection is the shorter test turnaround time and probably the increased true sensitivity of detection of certain pathogens.
Microbial DNA was measured using NAT-based pathogen detection with multiplex PCR testing for 45 targets (VYOO®; SIRS-Lab, Jena, Germany).
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