Sentence examples for multiplex mutation detection from inspiring English sources

The distinct advantage of FMCA lies in its capability of multiplex mutation detection [27].

10 A multiplex mutation detecting kit using Luminex technology (Mebgen Rasket Kit; Medical and Biological Laboratories, Nagoya, Japan) has been approved for the simultaneous detection of 48 types of RAS mutations.

Here, we combined the JAK2 V617F and MPL W515L/K mutation detection with a multiplex system, which reduced the total processing time of molecular diagnosis of MPNs.

The paradigm shown by adding sequential addition of samples for unique unigenes, unigene sequence coverage, and depth suggests that higher multiplexing can be done during library preparation while cutting the cost of mutation detection to redistribute the number of reads towards coverage rather than depth.

The application of multiplex PCR protocols further assists in monitoring problems of contamination and ADO in addition to mutation detection, making these assays highly accurate.

To avoid these potential causes of diagnostic errors, therefore, the current standard of practice is to combine targeted haplotyping of the gene locus by multiplex amplification of one or more closely linked or intragenic informative polymorphic markers with or without direct mutation detection.

To establish a comprehensive approach for mutation detection of PMS2, we have designed a strategy combining targeted capture next-generation sequencing (NGS), multiplex ligation-dependent probe amplification, and long-range PCR followed by NGS to simultaneously detect point mutations and copy number changes of PMS2.

The dual-labeled, self-quenched probes offered unprecedented combined advantage of enhanced multiplexing, improved flexibility in probe design, and expanded cross-platform compatibility, which would substantially improve FMCA in mutation detection of various applications.

A mass spectrometry (MS) based multiplex genotyping method using solid phase capturable (SPC) dideoxynucleotides and single base extension (SBE), named the SPC SBE, has been developed for mutation detection.

Quantum dot-mediated separation-free assay for point mutation detection.

The mutation detection reactions were performed in a total volume of 10 µl, containing 1 µl SAP/ExoI treated PCR product, 2.5 µl SNaPshot Multiplex Ready Reaction mix, 1 x Big Dye sequencing buffer and 1 µl probe mix.