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Moreover, all isolates with similar ICE elements (except one) clustered in the same branch, which indicates multiple transfer events of the HPI-ICE to other species.
This suggests multiple transfer events from individual donor types or from donors with similar genome signatures.
While multiple transfer events appears complex, there are well-known precedents for gene transfer between Prochlorococcus strains, facilitated by cross-infecting phage [ 17- 19].
Depending on the extent of gene order conservation around the HTgene between genomes it may be possible to distinguish single from multiple transfer events (Fig. 2A and 2B).
For three clades (clade I clade II and clade III, Fig. 3), this implies either multiple transfer events from one donor or a single acquisition event followed by dispersion of the acquired fragment into multiple regions of the host genome thereafter.
Although previous studies have already suggested horizontal transfer of Bov-B LINEs between squamate reptiles and mammals (Kordis and Gubensek 1997, 1998a, 1998b), our analysis suggests the possibility of multiple transfer events into and/or out of squamate genomes (supplementary fig. S5, Supplementary Material online).
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In contrast to the widely accepted scenario which assumes vertical transfer of GapC1 genes throughout chromalveolate evolution, we speculate that (i) there was a common ancestor of stramenopiles and alveolates as the "innovator" of the original GapC1 gene, and (ii) multiple lateral transfer events have taken place in GapC1 evolution.
Neither of these findings is unique to the Euglenozoa [4], [8], [9]; however, we also show that at least part of this complexity is best explained by differential loss of EFL and EF-1α from an ancestral state of co-occurrence rather than from multiple lateral transfer events.
This pattern is inconsistent with multiple horizontal transfer events into salamander genomes during the relatively recent past, corroborating the results from our phylogenetic analysis.
Specifically, multiple gene transfer events from cyanobacteria and chlamydiae (i.e. obligate intracellular pathogens AKA 'energy parasites') led to the evolution of chloroplast recAs.
Regarding recA, we infer multiple gene transfer events from cyanobacteria leading to the evolution of chloroplast recA, in accordance with the origin of chloroplasts from cyanobacterial ancestors [ 32].
Related(14)
several transfer events
multiple transmission events
numerous transfer events
multiple transposition events
many transfer events
multiple recombination events
multiple transfer approaches
multiple introduction events
multiple transfer functions
multiple transfer processes
multiple transfer programmes
multiple fireball events
multiple campaign events
multiple mutation events
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