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The RILs were planted in a simple lattice design with multiple replicates in a plot at Jiangpu Station, Nanjing Agricultural University, Nanjing, China.
Second, RVD2 handles multiple replicates in case samples.
To ascertain HTS compatibility and robustness, we examined the performance of the FRET assay in multiple replicates in 384-well plates.
Five thousand Calu-1 cells were seeded as multiple replicates in a 96-well plate and the next day treated with 5 μmol/L curcumin for 72 h.
To quantify the circuit response time, we calculated τ using data from multiple replicates in three individual experiments by first normalizing trajectories to the end point fluorescence of the L1+L2 condition to account for variation in fluorescence output between experiments (Supporting Information Figure S6).
To quantify the circuit response time of the SIM, we calculated τ using data from multiple replicates in three individual experiments by first normalizing trajectories to the average of the t = 0 fluorescence of each individual colony's with and without theophylline condition.
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Many interspecific crosses were represented by multiple replicates, and in these cases, we calculated the mean for each measure of reproductive isolation and used these mean values in subsequent analyses.
In the case of sample that is insufficient to carry out a proteomic study with multiple replicates, like in the case of an early stage breast tumor, does the information obtained from a single gel replicate still provide insight into the predominant cell signaling pathway at work in a cell?
Rather irregular, yet consistent in multiple replicates carried out in this study, were responses of CCNG1, CCNG2, BAX, BBC3 and TXN genes.
The multiple replicates permitted calculation of in-group variability, crucial for the proper implementation of 2-way (transcription of individual genes) and 3-way (gene-gene links in the network) ANOVA.
Amplification, probe preparation and hybridization protocols were performed as previously described [9] and multiple replicates were examined in each condition.
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