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Group I mGluRs, including mGluR1 and mGluR5, have long intracellular C-terminal tails containing multiple protein binding domains and sites for phosphorylation and ER retention.
This distortion is required to accommodate multiple protein binding events.
APC is large protein comprising 2843 aa and multiple protein binding sites.
RACK1 has multiple protein binding partners and affects a variety of cellular functions.
This indicates that a particular length of DNA containing multiple protein binding sites dispersed within the 2.5 kb XB fragment is required for proper insulator function and that insulator activity is a consequence of complex DNA-protein interactions (Figure 2).
Although we have failed to observe any appreciable cell cycle function of the Rbf1N domain by itself, we nevertheless propose that this domain plays an important function by creating multiple protein binding configurations and by tethering Rbf1 to chromatin.
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Greater specificity and affinity of multiple proteins binding to DNA in comparison with binary protein-DNA interactions were observed.
Strategies to identify multiple RNAs binding an individual protein of interest have advanced further than those designed to identify multiple proteins binding a given RNA.
The capacity to rapidly identify multiple proteins binding to an individual RNA of interest will allow characterization of the molecular mechanisms of action and the functional role of specific RNAs in human disease.
On the other hand, Hidden Markov Models, which can be thought of as one dimensional statistical mechanics models of multiple proteins binding on DNA, have been applied to study multiple binding sites for protein complexes in a stretch of DNA [ 38, 39].
RACK1's scaffolding properties are mediated by the presence of seven WD-repeats [ 46, 47] (see also sections 2.2 and 2.3) that present multiple protein-binding sites and facilitate interaction with specialized protein docking modules, including SH2 domains (Src and Fyn) [ 48, 49], plextrin homology (PH) domains (dynamin and p120GAP) [ 50, 51] and C2 domains (PKCs) [ 43, 45].
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