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Exact(9)
The original 430v2 probeset definitions provided multiple probesets for a single gene, typically targeting different isoforms, including mutually exclusive isoforms.
For the Affymetrix platform, we used the annotation provided by the manufacturer, version 25, which allowed us to identify single or multiple probesets for 105 of the 108 DM signature genes.
Multiple probesets for the same gene or transcription unit are summarized by the median expression value.
Multiple probesets for the same gene are summarized by the median expression value.
Multiple probesets for a gene were then collapsed to 1 entry per gene, based on the mean best-expressed probeset for that gene.
The Affymetrix U133A, U133 Plus 2.0 and U95AV2 arrays have different design criteria that may lead to the creation of multiple probesets for the same gene [ 33].
Similar(51)
Where multiple probesets were available for a given gene, the probeset with the best target region overlap with the nCounter target region was chosen.
When multiple ProbeSets were present for a single Entrez Gene ID they were collapsed by selecting the ProbeSet with the minimum p-value.
In cases where multiple probesets were available for a given gene, the probe set having the maximum present call percentage across the complete database was considered.
Importantly, using the gene name as an identifier, it is clear that multiple probesets are present for many genes but that these replicate probesets may not always generate similar detection rates.
In terms of pathway analysis we are interested in specific genes, so when we assessed the percentage of probesets that coded for a single gene name, we found that the Colorectal DSA identified many more individual genes than the Plus2.0 array, which identified multiple probesets that coded for the same gene name.
Related(20)
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