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Exact(29)
The array was evaluated using multiple primer pairs.
Multiplex RT-PCR uses multiple primer pairs to amplify the specific targets simultaneously from a single sample.
Multiple primer pairs for each gene amplifying 100 150 bp fragments with primers spanning exons were tested for relative efficiency using log dilutions of cDNA.
Multiple primer pairs for each gene were tested and the most suitable primer candidates were chosen by assessing the specificity of the PCR product and efficiency.
Multiplexed PCR reactions provide a potential solution to these problems, because multiple primer pairs amplify different parts of the genome together in a single reaction tube[33].
In contrast to the HPV typing where only a single primer pair was used to amplify a single target, multiple primer pairs are often used to amplify multiple targets in multiplex assays.
Similar(31)
It was therefore necessary to test multiple primer pair combinations for some targets, rejecting those that generated profile collapse or extensive plateau drifting as indicated by LRE analysis (see [ 18] for details about these anomalies).
Due to lack of detection of ovarian aromatase with real-time PCR, further examination of cyp19a was performed using multiple primer pair with visualization of expression using both real-time methods and 2% agarose gel/ethidium bromide.
Multiple primer-pairs were designed based on sequence data for Seg-2, targeting each of the seven EHDV serotypes (Table 1 and Table S1 - supporting information).
To determine whether gene duplication followed by recombination (an intralineage process) may explain the phylogenetic conflict in the Silene SlX1/Y1 gene, we use a novel probabilistic, multiple primer-pair PCR approach.
We then showed that MCPC could be performed in a multiple-primer-pair format through a novel primer dimer alleviating strategy.
Related(19)
several primer pairs
numerous primer pairs
many primer pairs
multiple primer combinations
various primer pairs
multiple system pairs
multiple centriole pairs
multiple sample pairs
multiple microphone pairs
multiple primer initiations
multiple nanocylinder pairs
multiple primer sequences
multiple primer sets
multiple transceiver pairs
multiple reactant pairs
multiple keypoint pairs
multiple receiver pairs
multiple word pairs
multiple gene pairs
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