Sentence examples for multiple primer sets from inspiring English sources
It should be noted that the use of multiple primer sets biases the number of sequences belonging to the identified taxa.
The combination of 16S rRNA gene amplification using multiple primer sets and the subsequent sequencing of the PCR products by Roche 454 pyrosequencing should therefore be a powerful method to assess the diversity of the microbiota in the GI tract of common carp.
The 5′RACE approach avoids the potential bias associated with the use of the multiple primer sets required to amplify all V region sequences and takes advantage of the conserved sequences offered by TRAC for TRA and TRBC1 and TRBC2 (96% nucleotide sequence identity) for TRB1.
These multiple primer sets were included to address fragmentation.
No dsrAB transcripts were detected from surface sediments outside the mat, despite nested amplification with multiple primer sets.
Since our RT-PCR product would detect transcripts derived from both promoters, multiple primer sets were designed to investigate expression from each mouse promoter.
To overcome these limitations, a multiple primer set assay including minimal degeneracies was developed for pcrA genes.
PCR was performed in a final volume of 10 μL containing 1× HotStarTaq buffer, 3.0 mM Mg2+, 0.3 mM dNTP, 1 U HotStarTaq polymerase (Qiagen Inc. USA), 1 μl DNA template and 1 μl multiple primer set.
In other single-marker SNP genotyping methods, where multiple primers sets designed with similar melting temperature are present in the reaction, undesirable primer-primer interactions and non-specific amplification from the genomic template can result unless careful consideration is given to primer design and assay optimisation.
The design of multiple, specific primer sets and assay optimization pose major challenges [ 22].
RT-PCR analysis with multiple NOTCH3 primer sets spanning the complete transcript, showed that the complete human NOTCH3 cDNA was present in the transgenic transcript (Additional file 1: Figure S2).
