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The differentiation potential of SOX9+ BCs was further confirmed by qPCR analysis of multiple marker genes with human specific primers.
To examine the requirement of the TERT gene during developmental hematopoiesis in zebrafish, the expression of multiple marker genes for both primitive and definitive hematopoiesis was analyzed by in situ hybridization.
Eventually, it may become routine to use the entire 16S gene, multiple marker genes, or even entire genomes.
Taken together the results support inductions of multiple marker genes for osteogenesis, angiogenesis, and hematopoiesis by severe hypoxia that may predispose cells to these lineages.
For shotgun metagenomics studies, use of strain-specific genes or use of phylogenetic information across multiple marker genes may also provide necessary discrimination.
The analysis of a single gene presumes that the gene history reflects that of the species, as opposed to the study of multiple marker genes.
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To date, the only reporters that achieve this without the need to sequence breakpoints are traffic light reporter (TLR) systems that elegantly combine multiple fluorescent marker genes to detect mutNHEJ and GT (25) or mutNHEJ, GT and SSA (26).
Results showed that palladin expression was rapidly induced during the differentiation of SMCs, and induces expression of multiple SMC differentiation marker genes by interacting with the MRTF complexes, which directly or indirectly bind to the CArG elements in the promoter regions of SMC marker genes.
This exquisite lineage restriction was particularly surprising in light of the expression of multiple pan-mesodermal marker genes, such as LHX1, MESP1 and MIXL1.
Because the presence of multiple markers per gene provided redundant data for mapping, the marker with the least missing data were selected for the gene, resulting in a final set of 4195 markers for mapping.
The latter has evolved from a single transcript approach to a multiple gene screen – 51 marker genes – that are closely correlated with neuroendocrine tumor biology [ 79] and overlap with genes e.g., APLP1 family, PNMA2 and CD59, in the current study.
Related(20)
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