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Multiple internal standards were used to correct for machine drift that was less than 2% across the mass range.
A single or multiple internal standards are generally applied for reducing the variability that may be introduced in the system during sample preparation.
A protocol for the analysis of multiple prostaglandins and leukotrienes in cell culture media by using multiple internal standards was validated.
The normalization was performed using multiple internal standards as described in the Supporting Information Text S1.
Furthermore, method performance was carefully monitored using multiple internal standards (5 to 10 depending on method, including analogues, 2H and 13C labeled metabolites) and duplicate analysis of samples.
Similar(55)
Internal standards guaranteed standardized measurements.
Hormones were analyzed by HPLC-MS/MS using multiple reactions monitoring and deuterated internal standards for each of the hormones and metabolites.
Individual lipid species were quantified by referencing to spiked internal standards using multiple reaction monitoring (MRM) transitions [ 76].
Image analysis and statistical calculations were performed using the Progenesis SameSpots software (NonLinear Dynamics, Newcastle, UK) and the 'Multiple stains per gel without internal standards' comparison method.
Stable isotope labeling with amino acids in cell culture (SILAC) is an optimal method for accurate quantitative proteomics and the use of SILAC has been expanded for tissue proteome quantification by using a mix of multiple SILAC-labeled cell lines as internal standards (super-SILAC) [ 15- 17].
A literature search revealed just two compounds that were used as internal standards during mass spectrometric determination of multiple artificial sweeteners; deuterated sucralose and sodium warfarin.
Related(20)
several internal standards
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multiple international standards
multiple local standards
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