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Propagating the library for multiple generations with drug concentrations that moderately inhibited the growth of the isogenic parental strain caused the abundance of strains with even minor fitness advantages or disadvantages to change measurably and reproducibly.
Typically QTL studies of this type require multiple generations with the use of inbred or hybrid lines.
Furthermore, many studies spanned multiple generations, with the median study duration at 34 years, and the maximum at 91 years (Fig. 4).
Thus cultures can be expanded and recloned for multiple generations, with the percentage of single cells giving rise to new colonies varying between cell lines (Fig. 1c).
Biological materials consisted of a parental susceptible strain together with three child strains selected across multiple generations with three insecticides from different classes: the pyrethroid permethrin, the neonicotinoid imidacloprid and the carbamate propoxur.
The BTG-silent lines had been crossed for multiple generations with a stock that is polymorphic relative to the inbred line B73, enabling B73 to be used as the outcross parent for constructing mapping populations.
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Recently, we identified several families of multiple generations affected with atrial fibrillation with no other underlying cause detected.
Nevertheless, including a polygenic effect is beneficial for a random mating population when estimated marker effects are used to predict GWEBV over multiple generations, especially with respect to the bias.
To experimentally estimate a traits heritability, mean phenotypic change (ΔZ) could be measured following selection over multiple generations, but as with experimental evolution, this is practical mostly for organisms with short generation times (days to weeks, Lohbeck et al. 2012).
We demonstrate that the underlying mechanism of this inability to complete differentiation is a progressive deregulation of gene expression over multiple cell generations, with multiple developmental pathways involved in hematopoietic stem cell specification and myeloid differentiation being affected.
One of the leading figures in 20th- and 21st-century art, Louise Bourgeois inspired multiple generations of artists with her unique and ever-evolving practice.
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