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The method precision evaluated the ability of the method to analyze multiple dilutions of sample.
The insert-to-insert variability applying a single dilution airflow (0.5 L/min) or multiple dilutions of CS was measured with WST-1 (Roche Applied Science, Rotkreuz, Switzerland).
Multiple dilutions of CS smoke were performed to determine the carbonyl concentration at dilution airflows of 0.1, 0.2, 0.5, 1.0, 1.5, 2.0, and 3.0 L/min.
In most microfluidic devices, single or multiple dilutions of reagents are required to perform reactions or measurements over a range of concentrations using a set of sample solutions to fill the inlets.
GAPDH cDNA was evaluated by performance of a GAPDH PCR on multiple dilutions of each cDNA sample.
We performed subretinal injection of multiple dilutions of the AC-GFP nanoparticles and assessed retinal expression by qRT-PCR (Fig 8).
Similar(46)
The IC50 values were determined on addition of multiple dilution of Ir.Cr (i.e. 0.5, 0.25, 0.125, 0.0625, 0.0313, 0.015 mg/ml) and eserine (i.e. 0.5, 0.25, 0.125, 0.0625, 0.0313, 0.015 mM) to the reaction mixtures and data obtained was analyzed on EZ Fit Enzyme kinetics software (Perrella Scientific Inc. Amherst, USA) decrease in absorbance indicated increase in enzyme inhibitory activity.
Four different BSA concentrations (5, 1, 0.5, and 0.1 μM) were obtained by multiple dilutions from a 15-μM mother solution of BSA in PBS buffer.
Samples from pooled fibers and whole muscles (same RNA control of microarray experiments) were amplified from multiple serial dilutions of the cDNA input.
Multiple serial dilutions of a reaction were fractionated alongside the undiluted reaction, and the substrate band was quantified from those that fell within the linear range of estimation.
The accuracy with which the individual TTP estimates fit the log-linear calibration line can be used to estimate assay measurement error (the intra-assay variability, σ) by fitting a hierarchical mixed-effects model to multiple dilution series (allowing estimates of β0 and β1 to vary among experiments to determine whether this significantly improves the fit of the model).
Related(20)
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