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We suggest that future studies should consider the use of multiple cores from each site to examine the within-site variability of the decline of S. austinii.
Samples from 133 of the patients were made into a tissue microarray (TMA) containing multiple cores from normal/benign regions (>3), prostatic intra-epithelial neoplasia (PIN) and at least two distinct regions of tumour for each patient.
Multiple cores from each patient were used.
Scores from multiple cores from each tumor across the two observers were averaged [ 5].
Multiple cores from the same patient were handled separately for RNA extraction.
For the lumican staining, we used the highest score of multiple cores from each tumor for further analysis.
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Mean number of vessels/core averaged over multiple cores for each individual tumour ranged from 0 to 48.5 vessels/core.
When averaged over multiple cores for each tumour, proliferation index ranged from 0 to 29.3% with a mean of 685 cells counted per tumour.
Dr Pinfold says one solution his team is exploring is to use multiple cores, switching from one to another not to increase speed, but rather to minimise the total heat generated.
As another aspect, because MMIHC is essentially complementary to TMA (i.e., multiple channels connected on top of a tissue slide rather than multiple cores taken from different tissue samples on a single slide), it may compensate for limitations associated with the latter method.
The GEE method takes into account the association among the multiple cores obtained from the same patient through modeling a working correlation matrix.
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