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The experiments reviewed here include reactions of single and multiple capture and ionization induced by keV proton to GeV/u U92+ impact, electron and antiproton impact ionization as well as single and double ionization by photoabsorbtion and Compton scattering from threshold to 100 keV.
The sequences for multiple capture and intermediate oligonucleotides per target gene were designed and prepared as described previously (23, 24 ).
Our results demonstrate that selection of target specific capture sequences is critical for performance of this assay, and the use of multiple capture and intermediate oligos is critical for detecting a wide variety of strains with the desired sensitivity.
We studied the capacity of the beads after multiple capture and release cycles by using a constant amount of the three PCR products as input for each iterative purification cycle.
Designing multiple capture and intermediate oligonucleotides with sequences covering the entire genome ensures specific capture of multiple target genes on the nanomicroarray and subsequent detection with a universal nanoparticle probe regardless of mutation, deletion, and influenza reassortment.
Another possible reason for higher detection sensitivity is the use of multiple capture and intermediate oligos to detect a target in multiple regions, which significantly increases the binding of gold NPs and could overcome the diagnostic challenge posed by potential gene mutations.
Similar(53)
To help understand the approach to the decay asymptote, which is established through multiple capture-and-release cycles, a qualitative model was proposed.
Alternatively, the sensitivity of TB detection in an infected badger might be increased by multiple captures (and testing) of that animal.
This is considered akin to multiple captures and recaptures in animal abundance studies (5, 6).
Also, application of multiple capture probes and complementary oligonucleotides across gene locus is essential to prevent catalysis of nc-rRNAs.
The disadvantage is the requirement for specialized microscopy, visualization of tissue specimens through multiple filters, multiple image capture, and the relatively short half-life during which signal is visible.
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