Assembling the full transcript required contigs from multiple assemblies, and only a subset of the individual assemblies contained sequences fragments for the middle of the transcript.
The quality filtered sequence reads were assembled using the GS de novo sequence assembler v.2.5.3 (Roche 454 Life Sciences, Branford, CT, USA) and multiple assemblies were performed with modified parameters (i.e. adjusting minimum overlap length).
Close paralogues, particularly when tandemly arrayed, and subtle differences in the structure of gene models across multiple assemblies of the same genome sequence present significant challenges.
Buildings are complex products containing relatively large numbers of distinct parts that are collected in multiple assemblies for different design, analysis and production purposes.
However, results described here are based on patterns observed in multiple assemblies.
Multiple assemblies were conducted to calibrate the optimum kmers parameters.
