The Parliament of 1295, which included representatives of shires, boroughs, and the lesser clergy, is usually styled the Model Parliament, but the pattern varied from assembly to assembly, as Edward decided.
PCR was used to confirm the sizes of the non- D1 genes and to validate the region that included the D1 genes, which varied among assemblies.
The 5' end of the D1-y gene, which was a region that varied among assemblies, was also sequenced at 2.94× coverage with gene specific primers (Table 1) and results for a correct D1-y gene were not consistent with assemblies 8 and 10, which were also eliminated.
Surprisingly, telomerase reconstitutions yielded heterogeneous DNA-bound TERT monomer and dimer complexes in relative amounts that varied with assembly and purification method.
According to the completeness assessment by CEGMA, the completeness scores of the integrated assemblies varied substantially even though these assemblies were produced from the same set of sequence reads (Fig. 3c and Additional file 6).
Fosmid coverage was seen to vary between assemblies, particularly in snake, but was not correlated with genome assembly size (Additional file 2: Figure S8), reinforcing the notion that overall assembly size is not necessarily a good predictor of assembly quality.
Probes from the initial run based on the old assembly were re-mapped to the new assembly in order to identify loci affected by deletion/duplication status that vary by assembly map.
The morphology of the assemblies varied with the concentration of TPPtFFC.
The contiguity of these assemblies varied from pool to pool with contig N50 ranging from 10.8 (pool 6) to 19.9 kb (pool 1), and scaffold N50 ranging from 242 (pool 5) to 518 kb (pool 1).
The tracks followed by APP-VP26-GFP assemblies varied (Figure 8B).
The sizes of the six assemblies varied from 97.9 Mb to 151.4 Mb.
