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Exact(5)
For phylogenetic analysis of the Cox proteins, TBLASTX searches were carried out, the corresponding sequences recovered from GenBank and a multiple alignment generated using Clustal X and manually refined as mentioned above.
Nucleotide sequences of all HPIV-4 strains were determined and entered into a multiple alignment generated by the Clustal W software (version 1.83) (1.83.
Analysis of a multiple alignment generated using the sequences identified by the searches shows that the capsid protein has lost the catalytic residues necessary for the nuclease activity.
The sequence region comprising nucleotides 60-708 of the F gene was entered into a multiple alignment generated by Clustal-W and corrected through final visual inspection with the SeqLab application (Wisconsin package version 10.3, Accelrys Inc., San Diego, CA).
The VP1/VP2 nucleotide sequences from this study, as well as prototype sequence type (ST 1 and ST2 (1 ), were entered into a multiple alignment generated by ClustalW software version 1.83 (www.molecularevolution.org/software/clustalw) and corrected through final visual inspection with the SeqLab application (Wisconsin package version 10.3; Accelrys, San Diego, CA, USA).
Similar(55)
The sequences were then provided to Clustal W and the multiple alignments generated were used as input for the RNAz software.
We refer to the set of local multiple
Multiple alignments generated for each distinct cluster were evaluated for the presence of common sequence motifs.
Phylogenetic clustering was also performed using Maximum Likelihood and Maximum Parsimony methods in MEGA 6.0 [ 62] with multiple alignments generated using MUSCLE (http://www.ebi.ac.uk/Tools/msa/muscle/).ac.uk/Tools/msa/muscle/
In the case that multiple alignments generated by CLUSTALW were used for phylogenetic inference, regions that could not be aligned unambiguously or containing deletions/insertions had been removed prior.
The determination of the consensus sequences of each repetitive sequence and their classification were carried out by manual inspection based on the multiple alignments generated by the ClustalW program.
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