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The multiple alignment obtained in this section will be further used to find structurally conserved RNAs using RNAz.
These sequences were then added to the multiple alignment obtained by Lavire et al. [ 13], and a NJ phylogenetic tree was inferred (Fig. 3).
Each was run on the MultiZ 43-species multiple alignment obtained by removing the human sequence from the alignment, so that human conservation/mutations do not affect the scores produced.
Degenerate forward and reverse Hr-specific primers were designed according to an amino acid sequence multiple alignment obtained from the Hr sequences available in the Swiss-Prot database: Phascolopsis gouldii (P02244), Themiste zostericola (P02245), T. dyscriptum (P02246), and Siphonosoma cumanense (P22766).
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We extracted the amino acids localized in the stored positions within each sequence by considering the multiple alignments obtained as reference.
Fig. 2C presents the multiple structure/sequence alignment obtained with the 3DCoffee web server [39].
The multiple sequence alignment obtained in this way was used as the reference for the structural prediction of hTAS2R38 by homology modeling.
The plant myosin sequences were added to the structure-guided multiple sequence alignment obtained from [ 23].
We also tested the tools on a multiple sequence alignment obtained from our study on the Drosophila X chromosome.
Let X be a set of n taxa (i.e., objects, species) and A be a multiple sequence alignment obtained for the taxa from X.
A comprehensive test on different datasets including Rfam sequence alignments and a multiple sequence alignment obtained from our study on the Drosophila X chromosome reveals that RSpredict is competitive with the existing tools on the tested datasets.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com