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This reduced LD requires much higher marker density than family-based mapping.
The much higher marker density achieved in our study allowed for the detection of more COs, especially COs separated by shorter distances.
However, while this work represents a substantial advancement in SNP development for tall fescue, a much higher marker density is anticipated to be required for both GWAS and GS applications.
We employed a pooled hybridization approach for expediency, allowing use of a genotyping array with much higher marker density than that of previous surveys for introgression in West Africa (Marsden et al. 2011; Weetman et al. 2012).
The inversions identified here are of this size (3-4 Mbp) and a much higher marker density (up to 20 markers per Mbp in the present study) is needed to reliably identify recombination suppression at this genomic scale.
Compared to previously reported Cucurbita maps [ 13, 38– 40, 62– 65], this map had much higher marker density and fewer gaps, making it the most saturated genetic map in Cucurbita species reported to date.
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However, this assumes uniform marker spacing and LD intensity, whilst in reality much higher local marker density may be required for some of these regions.
In the present work, the density of the SNPs is much higher (61,144 markers) and the selection of them based on buffaloes data.
In zebrafish the RH map allowed much higher gene-marker coverage of the genome and permitted comparisons with other vertebrates [ 19, 20].
The practical consequence of this much higher efficiency of marker replacement is that in gsSSG mutagenesis recombinants can be isolated by streaking colonies directly from transformation plates to FOA medium (see Methods).
Compared with JoinMap, MergeMap tended to generate integrated maps with much higher consistency of marker order compared with each population-specific map, with Spearman's correlation coefficients >0.95 across all LGs for all three populations (Table 3).
Related(20)
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