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The complexity and low abundance of pyrene degrading communities together with limited label incorporation are preventing the use of other less sensitive techniques, like nucleic acid-SIP that give much more detailed information on the identity of individual degraders compared to PLFA-SIP but require much higher label incorporation into biomolecules.
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Probe 5S-1, which showed a much higher labeling efficiency on U. prolifera, was ultimately selected as the probe for the FISH detection.
The results are given in Figure 3B and indicate a much higher labelling enrichment in the upper part of the glycolytic pathway and the pentose phosphate pathway (PPP).
As shown in Figure 4B, intraepithelial CD8+ T cells showed a much higher labelling index for Ki-67 (7% in median) than peritumoral CD8+ T cells did (1% in median), indicating presumable tumour-specific reactivity of intraepithelial T cells.
First, we optimized the chemical composition of the deuterated culture medium to achieve a much higher deuterium labeling efficiency and improved imaging sensitivity and speed of our SRS instrumentation.
We found that CLAcrBW13C/P223G was consistently labeled to a much higher level than CLAcrBW13C in all trials that we performed.
Overall there was a much higher density of labeling for both Aβ and dityrosine in AD CSF compared to controls, highlighting the presence of Aβ and also showing the increased level of dityrosine crosslinked proteins in AD CSF.
The much higher portion of labeled endothelial cells found in our study may be due to the higher sensitivity of hPLAP histochemistry compared with the in situ hybridization technique necessary for visualization of the globin/pBR322 genomic marker, or due to species differences.
The alcohol content may be much higher, however, and labels must be checked to determine the alcohol content.
As with AT8 labelling above, the K3 group showed a much higher average 4-HNE labelling intensity and area than the WT group (fivefold and 20-fold, respectively) and this labelling was significantly reduced (by 50% and 80%, respectively) in the K3-NIr group.
However, two younger recently settled FPs appeared outliers with much higher rates of the label 'unaware or no recent contact' than other FPs and also relatively high rates of 'possible cognitive impairment or dementia'.
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