Sentence examples for mouse zygote to from inspiring English sources

Briefly, we microinjected pRP.ExBi-nestin-Dmp1-IRES-eGFP DNA into mouse zygote to overexpress Dmp1 with nestin promoter.

Briefly, the authors analysed the development of the mouse zygote to the bastocyst by measuring gene expression on a single-cell level.

The benefits of our approach are illustrated at the example of gene-expression data from the mouse zygote to the blastocyst, as presented in (Guo et al., 2010).

Results: We present a novel framework based on Gaussian process latent variable models (GPLVMs) to analyse single-cell qPCR expression data of 48 genes from mouse zygote to blastocyst as presented by (Guo et al., 2010).

In this article, we present our new approach at the example of the cellular development of the mouse zygote to blastocyst; however, our methodology is not limited to this particular example and can be applied to a range of other datasets and cell types.

Furthermore, we demonstrated that the FnCas9-ribonucleoprotein complex can be microinjected into mouse zygotes to edit endogenous sites with the 5′-YG-3′ PAM, thus expanding the target space of the CRISPR-Cas9 toolbox.

This strategy has been successfully used in mouse zygotes to generate indels and knock-in alleles (Ran et al. 2013a; Fujii et al. 2014).

HMGB1 is indeed passively released from nuclei upon cell death and actively secreted as a cytokine (29), and the addition of recombinant HMGB1 into culture medium enhances in vitro development of mouse zygotes to the blastocyst stage in the absence of BSA supplementation (30).

Nevertheless, the Fe-S radical S-adenosylmethionine (SAM) domain of elongator complex protein 3 (Elp3) was shown to be involved in paternal DNA demethylation in the mouse zygote leading to the hypothesis of a direct radical SAM-mediated demechanismon mechanism.

Likewise, TBP protein only accumulates in 2-cell mouse zygote, at the onset of mouse zygotic gene activation [ 50, 51].

One of the first single cell studies using microfluidics platforms analysed cells from the mouse zygote through to the 64-cell stage blastocyst 43.