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The femoral and spinal bones of mouse were scanned at an isotropic voxel size of 27 µm (80 kV, 450 µA and 2000 ms integration time) using the eXplore Locus micro-CT scanner (GE Health Care).
Control and emphysema mouse were scanned 21 days, fibrosis mouse 14 days after application.
Bilateral distal femurs and midshaft femurs of each mouse were scanned with a high-resolution μCT scanner (vivaCT 40, Scanco Medical, Brüttisellen, Switzerland).
The lumbar and femoral regions of each intact mouse were scanned by using the Piximus Bone Densitometry system, as previously described [ 11].
Briefly, for μ-CT analysis, tibia isolated from each mouse were scanned by using a cone-beam microfocus X-ray computed tomography (μCT40; Scanco Medical AG, Brüttisellen, Switzerland), image acquisition was performed at 100 kV and 98 μA with a 0.9-degree rotation between frames.
Similar(54)
To achieve this, the mouse was scanned with an X-ray MicroCT (Siemens Preclinical Solutions, Knoxville, TN).
Each mouse was scanned in supine position with its head secured via ear and tooth bars.
The mouse was injected with 10 MBq of activity, and the mouse was scanned at 90 min after injection.
As this activity was too low for a short SPECT scan, the mouse was scanned for 4 h post-mortem in order to allow acquisition of sufficient counts.
An entire colorectal tumor implanted in an anesthetized mouse was scanned using the SSB scanning probe for 4 min. After each scanning, a scanning curve was obtained, as shown in the inset of Figure 2a.
Every mouse was scanned with a CT-phantom and isosurface was set in Hounsfield units according to the phantom which included hydroxyapetite, water and air.
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