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Regev's team comprehensively characterized rod bipolar cells (teal) and cone bipolar cells (orange) in the mouse retina using Drop-Seq, a technology they helped invent.
Our immediate goals are to define and characterize novel interneuron pathways in the mouse retina using optogenetic, electrophysiology and inactivation methods.
The present study was designed to examine the regulation of crystallin genes and protein in the mouse retina using the BXD recombinant inbred (RI) strains.
We have traced Aβ accumulation quantitatively in the ageing mouse retina using immunohistochemistry and Western blot analysis.
Due to the limited volume of sera from the MAR patients, we could not try immunostaining on the monkey or the mouse retina using the serum from the patients #8 and #23.
By transducing the Crb1−/− mouse retina using AAV2/6 vectors in combination with the GFAP promoter, we demonstrate that it is possible to specifically express transgenes in activated Müller glial cells, without expression in retinal ganglion cells, amacrine, bipolar, horizontal or photoreceptor cells.
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We investigated mouse retinae using the commercially available Spectralis OCT device with slight modifications.
Total RNA was isolated from mouse retinae using the RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.
Total mRNA was prepared from freshly dissected whole mouse retinas using the TRIzol reagent (Molecular Research Center, Cincinnati, OH).
Total RNA was isolated from rat and mouse retinas using the RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.
Total RNA was isolated from human blood lymphocytes and retina, and from mouse retinas using Tri reagent (Sigma Aldrich, St Louis, MO, USA) and treated with RQ1 RNase-free DNase (Promega Corporation).
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